Recombinant CD86 antibody

Catalog No. ABIN7072475

The Rabbit Chimeric anti-CD86 antibody has been validated for FACS, WB, IHC, Func and BP. It is suitable to detect CD86 in samples from Mouse.

Quick Overview for Recombinant CD86 antibody (ABIN7072475)

Target: CD86

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Functional Studies (Func), Blocking Peptide (BP)

Clone: PO-3

Product Details anti-CD86 Antibody

Purpose: Anti-CD86 [PO3], Rabbit IgG, kappa

Specificity: This antibody is specific for murine CD86, an ~80 kDa surface receptor of the B7 family of costimulatory molecules. In addition to CD80, CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used in multiple FACS experiments for various immunological applications, such as to demonstrate how the C-type lectin receptor DCIR modulates immunity to tuberculosis (Troegeler et al., 2017), to elucidate the mechanism of Th17 cell differentiation by cholera toxin (Kang et al., 2016), and to evaluate the role of the spleen tyrosine kinase in graft-versus-host disease (Flynn et al., 2015). This antibody has also been used in immunofluorescence to investigate the potential of peritoneal cavity B cells to alleviate murine colitis (Maseda et al., 2013) and in Western blot to study the effect of interleukin 4-induced gene-1 (IL4I1) overexpression on the expression of M2 markers and M1-associated cytokines (Yue et al., 2015). Furthermore, this antibody has been used, together with the anti-CD80 antibody, in numerous in vivo studies, such as in an NZB/W F1 mouse lupus model, to clarify the role of CD80 and CD86 in murine Ab-mediated autoimmunity (Nakajima et al., 1995), in graft bearing recipient BALB/c mice to evaluate the roles of CD80 and CD86 costimulatory molecules in corneal allograft rejection (Kagaya et al., 2002), and in transgenic BALB/c mice to demonstrate the critical contribution of CD80 and CD86 in anterior chamber-associated immune deviation (Tsukahara et al., 2005). This antibody has been used for IHC-F on mice salivary gland (Saegusa et al., 2000, PMID: 10632675), eye (Kagaya et al., 2002, PMID: 11878826), and ear (Nuriya et al., 1996, PMID: 8671681) (Ritprajak et al., 2008, PMID: 18461054) (Furusawa et al., 2019, PMID: 30978356) tissue.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background: PO.3, PO3.1, B7-2, Activation B7-2 antigen, B-lymphocyte activation antigen B7-2, B-lymphocyte antigen B7-2, B7.2, CD28 antigen ligand 2, CD28LG2, CD86, early T cell costimulatory molecule-1, early T-cell costimulatory molecule 1, ETC-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86

UniProt: P42082

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation