Recombinant LY6C + LY6G antibody

Catalog No. ABIN7072477

Product Details

Target: LY6C + LY6G

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Depletion (Dep)

Purpose: Anti-Ly6G/Ly6C [RB6-8C5], Rabbit IgG, kappa

Specificity: This antibody is specific for murine Ly6G, a a 21-25 kDa glycosylphosphatidylinositol (GPI)-linked protein expressed by the myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes express Ly6G transiently during bone marrow development, while Ly6G expression in granulocytes and peripheral neutrophils directly correlates with the cell's level of differentiation and maturation. This hallmark makes Ly6G a good marker for these particular cell populations. Ly6G has also been implicated in the development of antitumor responses.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994)

Clone: RB6-8C5

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used in FACS analyses, immunoprecipitation, immunoblotting, and immunohistochemistry in various studies, such as to elucidate the origin and roles of macrophages in the post-myocardial infarction remodelling (Sager et al, 2016), to investigate the pathogenesis of primary biliary cirrhosis (Yang et al, 2016), to confirm the phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice (Ricard et al, 2016), and to highlight an important ageing-related defect in the mucosal immune system's ability to sample lumenal antigens (Kobayashi et al, 2013). This antibody has also been used in vivo, for example, to deplete Treg cells when delineating the paralleled developments of inflammation and immunosuppression in tumor microenvironment (Yang et al, 2010), or to deplete neutrophils when underpinning a causal role for neutrophils in severe asthma (Wilson et al, 2009).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: LY6C + LY6G

Alternative Name: Ly6G/Ly6C

Background: Gr-1, Gr1, Ly-6G, ly-6G.1, ly6C

UniProt: P35461