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Recombinant CCRL2 antibody (AA 1-24)

The Rat Monoclonal anti-CCRL2 antibody has been validated for WB, ELISA, IHC, FACS, ICC, Func and IP. It is suitable to detect CCRL2 in samples from Mouse.
Catalog No. ABIN7072484

Quick Overview for Recombinant CCRL2 antibody (AA 1-24) (ABIN7072484)

Target

See all CCRL2 Antibodies
CCRL2 (Chemokine (C-C Motif) Receptor-Like 2 (CCRL2))

Antibody Type

Recombinant Antibody

Reactivity

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Mouse

Host

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Rat

Clonality

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Monoclonal

Conjugate

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This CCRL2 antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Functional Studies (Func), Immunoprecipitation (IP)

Clone

BZ5B8
  • Binding Specificity

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    AA 1-24

    Purpose

    Anti-CCRL2 [BZ5B8], Rat IgG2a, kappa

    Specificity

    This antibody is specific for murine CCRL2 (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites. CCRL2 presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23. Chemerin can block anti-CCRL2 antibody binding.

    Characteristics

    Original Species of Ab: Rat

    Original Format of Ab: IgG2a

    Purification

    Protein A affinity purified

    Immunogen

    This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate. The immunizing amino-terminal mCCRL2 peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1-24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).

    Isotype

    IgG2a kappa
  • Application Notes

    This antibody has been used in various FACS analyses, for instance, to confirm that it is only specific to mCCRL2-hemagglutinin (HA)/L1.2 transfectants and that peritoneal macrophages treated with LPS, TNFα, IFN-γ, or poly:IC up-regulate mCCRL2 protein expression (Zabel et al., 2008). Of note, this antibody binding to mouse peritoneal mast cells could be inhibited by chemerin, a protein ligand for signaling receptor CMKLR1 (Zabel et al., 2008). This anti-CCRL2 antibody can be used in wide variety of assays to study leukocytes and immune response. For example, this antibody has been used to examine the expression, regulation, and function of atypical chemerin receptor CCRL2 on endothelial cells (Monnier et al., 2012) and to delineate how the chemoattractant chemerin suppresses melanoma by recruiting natural killer cell antitumor defenses (Pachynski et al., 2012).

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    PBS with 0.02 % Proclin 300.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target

    CCRL2 (Chemokine (C-C Motif) Receptor-Like 2 (CCRL2))

    Alternative Name

    CCRL2

    Background

    CCR11, C-C chemokine receptor-like 2, Chemokine receptor CCR11, CKRX, CRAM-A, CRAM-B, G-protein coupled beta chemokine receptor, L-CCR, Lipopolysaccharide-inducible C-C chemokine receptor

    UniProt

    O35457
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