Recombinant CD40 Ligand antibody

Catalog No. ABIN7072505

The Hamster Monoclonal anti-CD40 Ligand antibody has been validated for FACS, Func and BP. It is suitable to detect CD40 Ligand in samples from Mouse.

Quick Overview for Recombinant CD40 Ligand antibody (ABIN7072505)

Target: CD40 Ligand (CD40LG)

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func), Blocking Peptide (BP)

Clone: MR1

Product Details anti-CD40 Ligand Antibody

Purpose: Anti-CD40L [MR1], Hamster (Armenian) IgG, kappa

Specificity: This antibody is specific for murine CD40 ligand (CD40L), a 39 kDa transmembrane glycoprotein. CD40L is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD40L-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.

Characteristics:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Armenian hamsters with murine activated Th1 (D1.6) plasma membrane as described by Noelle et al (1992).

Isotype: IgG kappa

Application Details

Application Notes: [Yesterday 2:23 PM] Victor Koolma This antibody was administred to SOD1 G93A mice, which exhibit ALS like symptoms, it was able to delay paralysis onset, it improved body-weight maintenance and extended survival (Lincecum et al., 2010, PMID:20348957). This antibody has been used in various FACS analyses for diverse immunological applications, such as to indicate how naive CD4 T cells constitutively express CD40L and augment autoreactive B cell survival (Lesley et al, 2006), to prove that the interaction between natural killer cells and dendritic cells has a pivotal role in the sensitization phase of contact hypersensitivity (Shimizuhira et al, 2014), and to demonstrate that enhanced CD8 T cell responses through GITR-mediated costimulation could resolve chronic viral infection (Pascutti et al, 2015). This antibody has also been used for in vitro and in vivo blocking and functional studies, for instance, to indicate that the 39- kDa CD40L membrane protein expressed on activated Th is a binding protein for CD40 and functions to transduce the signal for Th-dependent B-cell activation (Noelle et al, 1992), and to demonstrate that the CD40L-CD40 pathway can augment the survival of autoantigen-engaged B cells in the absence of T cell activation (Lesley et al, 2006). Furthermore, this antibody clone (MR1) has been re-formatted as a rat IgG2b version and used for in vivo functional assays to suggest that short pulses of anti-CD40L antibody therapy may still be useful in tolerance protocols even when the Fc region is disabled (Daley et al, 2008).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40L

Background: CD154, CD40 ligand, CD40-L, Cd40l, gp39, HIGM1, IGM, IMD3, Ly-62, Ly62, RP23-153G22.3, T-BAM, T-cell antigen Gp39, TNF-related activation protein, Tnfsf5, TRAP, Tumor necrosis factor ligand superfamily member 5.

UniProt: P27548

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints