Recombinant RHD antibody

Catalog No. ABIN7072535

The Rabbit Chimeric anti-RHD antibody has been validated for FACS. It is suitable to detect RHD in samples from Human.

Quick Overview for Recombinant RHD antibody (ABIN7072535)

Target: RHD (Rh Blood Group, D Antigen (RHD))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This RHD antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: D7C2

Product Details anti-RHD Antibody

Purpose: Anti-Rhesus D antigen [D7C2], Rabbit IgG, lambda

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgM

Purification: Protein A affinity purified

Immunogen: A Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.

Isotype: IgG lambda

Application Details

Application Notes: FC confirmed the binding of D7C2 to Rh D+ red cells in artifical mixtures (Edelman et al, 1997). The ADCC of D7C2 showed a percentage of 93 % lysis (Edelman et al, 1997). D7C2 has been expressed recombinantly using a baculovirus-insect cell expression system (Edelman et al, 1997).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for RHD

Target: RHD (Rh Blood Group, D Antigen (RHD))

Alternative Name: Rhesus D antigen

Background: CD240D, RHXIII, Rh polypeptide 2, RhPII