Recombinant LAG3 antibody

Catalog No. ABIN7072564

This Rabbit Chimeric antibody specifically detects LAG3 in IP, FACS and BP. It exhibits reactivity toward Mouse.

Quick Overview for Recombinant LAG3 antibody (ABIN7072564)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This LAG3 antibody is un-conjugated

Application: Immunoprecipitation (IP), Flow Cytometry (FACS), Blocking Peptide (BP)

Clone: C9B7W

Product Details anti-LAG3 Antibody

Purpose: Anti-LAG-3 [C9B7W], Rabbit IgG, kappa

Specificity: This antibody is specific for an epitope in the D2 domain of murine LAG-3.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein.

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used in flow cytometric analysis of splenocytes (Workman et al, 2005), T cell hybridomas expressing wild-type or chimeric CD4:murine LAG-3 (Workman et al, 2002) and CD4+ regulatory T cells (Chien et al, 2017). It has also been used in immunoprecipitation analysis of murine T hybridoma 3A9 cells transduced with murine LAG-3 (Li et al, 2004). This antibody blocks LAG-3 function in vitro, inhibiting antigen-induced IL-2 production in LAG-3-expressing T cell hybridomas, but does not block LAG-3:MHC class II interaction (Workman et al, 2002). Treatment with this antibody also blocks LAG-3 function in vivo, leading to accelerated homeostatic expansion of WT T cells (Workman et al, 2005). Administration of this LAG-3-blocking antibody, in combination with PD-L1 blockade, synergistically improves the survival of lymphodepleted myeloma-bearing mice (Jing et al, 2015).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: Lag3

Background: CD223, LAG3, Lymphocyte activation gene 3 protein

UniProt: Q61790

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints