Recombinant C5AR1 antibody (pSer332, pSer338)

Catalog No. ABIN7072652

The Mouse Monoclonal anti-C5AR1 antibody has been validated for WB and IF. It is suitable to detect C5AR1 in samples from Human.

Quick Overview for Recombinant C5AR1 antibody (pSer332, pSer338) (ABIN7072652)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C5AR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 32-G1

Product Details anti-C5AR1 Antibody

Binding Specificity: pSer332, pSer338

Purpose: Anti-C5aR [32-G1], Mouse IgG1, kappa

Specificity: The 32-G1 antibody recognizes C5aR which has its serine 334 phosphorylated. The mAb 32-G1 reacted specifically with a C5aR peptide that contained phosphoserine 334 and also reacted with C5aRs that were isolated from C5a-stimulated, transfected RBL-C5aR cells. It did not recognize a receptor mutant (C5aR-S-) with alanine replacement of all six C-terminal serine phosphorylation sites (Pollok-Kopp et al., 2007). Antibody 32-G1 specifically reacts with C5aR phosphoserine 334, independent of the phosphorylation state of the neighboring serine residues (Pollok-Kopp et al., 2007). Studies with Bt2cAMP-treated U937 cells show that 32-G1 specifically reacts with ligand-activated C5aR at endogenous receptor expression levels but only weakly recognizes C5aR isolated from unstimulated cells (Pollok-Kopp et al., 2007).

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice a synthetic C5aR C-terminal phosphopeptide that contained phosphoserine instead of serine at position 334 and also at the neighboring phosphorylation sites Ser-332 and Ser-338.

Isotype: IgG1 kappa

Application Details

Application Notes: The 32-G1 antibody is recommended for the detection and analysis of C5aR (CD88) phosphorylated on Ser-334. For instance, this antibody was successfully used in the identification of C5aR protein in a U937 cell lysate via Western blot (Pollok-Kopp et al., 2007).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Alternative Name: C5aR

Background: CD88, C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, pC5aR, 32G-1

UniProt: P21730

Pathways: Complement System