Recombinant CCR5 antibody (pSer337)

Catalog No. ABIN7072658

The Rabbit Chimeric anti-CCR5 antibody has been validated for WB, ELISA, FACS, IF and IP. It is suitable to detect CCR5 in samples from Human.

Quick Overview for Recombinant CCR5 antibody (pSer337) (ABIN7072658)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CCR5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: V14-2

Product Details anti-CCR5 Antibody

Binding Specificity: pSer337

Purpose: Anti-CCR5 (phosphoserine 337) [V14/2], Rabbit IgG, kappa

Specificity: V14/2 antibody has been demonstrated to exclusively recognise CCR5 (CD195) phosphorylated at Ser 337.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice with a synthetic phospho peptide corresponding to the C-Terminal region of human CCR5 conjugated to BSA.

Isotype: IgG kappa

Application Details

Application Notes: The V14/2 antibody is recommended for the specific detection and analysis of CCR5 phosphorylated on serine 337 (Pollok-Kopp et al., 2003). It was used in the detection of pS337 CCR5 in lysates of RBL-2H3 cells via Western blot (Pollok-Kopp et al., 2003). With other anti-CCR5 antibodies which react with nonphosporylated CCR5 (Ab01577) or CCR5 phosphorylated on different serine residues (Ab01578), it can be used to study dynamic regulation of CCR5 and of phosphorylation mechanisms.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CCR5

Background: CD195, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor, p337, pS337, Ser-337, Ser337

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size