Recombinant Cytokeratin 7 antibody

Catalog No. ABIN7072675

This Rabbit Chimeric antibody specifically detects Cytokeratin 7 in WB, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant Cytokeratin 7 antibody (ABIN7072675)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: OV-TL 12-30

Product Details anti-Cytokeratin 7 Antibody

Purpose: Anti-Cytokeratin 7 [OV-TL 12/30], Rabbit IgG, kappa

Specificity: This antibody recognizes cytokeratin-7. Cytokeratins are a subfamily of intermediate filament proteins but this antibody does not recognize other intermediate filament proteins. The antibody reacts with proteins that are found in most ductal, glandular, transitional, and biliary duct epithelial cells.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was originally raised by immunizing BALB/c mice with ovarian carcinoma cell line OTN11.

Isotype: IgG kappa

Application Details

Application Notes: Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometry. Cytokeratin 7 (CK7) labeling can help distinguish between lung, breast carcinomas, and urothelial carcinomas that typically stain positive, and colon and prostate carcinomas that typically lack CK7 expression (Jerome et al., 2004, Logani et al., 2003, Murray et al., 2004, Roy et al., 2011). CK7 is a common marker of primary lung adenocarcinomas (almost all cases) with a lower specificity since it is also observed in other primary lung carcinomas and non-pulmonary carcinomas (Jerome et al., 2004). Anti-cytokeratin 7 has also been useful in the differential diagnosis of ovarian neoplasms (McCluggage et al., 2005). Furthermore, this antibody is a marker for distinguishing between the conjunctival and corneal surface epithelia which may be used in the diagnosis of limbal stem cell deficiency (LSCD) (Jirsova et al., 2011, pmid: 21693612). Finally, OV-TL 12/30 has proven very effective and popular in immunohistochemistry, for instance, one group used it to label cytokeratin-7 in human term placental samples (König et al., 2014, pmid: 24326460).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: Cytokeratin 7

Background: CK-7, Keratin, type II cytoskeletal 7, Cytokeratin-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, CK7, CK 7

UniProt: P08729