Recombinant CD40 antibody (Extracellular Domain)

Catalog No. ABIN7072684

This Mouse Monoclonal antibody specifically detects CD40 in BR. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD40 antibody (Extracellular Domain) (ABIN7072684)

Target: CD40

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 antibody is un-conjugated

Application: Blocking Reagent (BR)

Clone: Chi220

Product Details anti-CD40 Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-CD40 [chi220], Mouse IgG2a, kappa

Specificity: This antibody binds the CD40 and blocks the humoral immune responses to T cell dependent antigens in a dose dependent manner.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was generated by immunizing female BALB/c mice with a fusion protein containing extracellular domain of human CD40 fused to the hinge CH2 and CH3 domains of a murine IgG2b antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody blocks the interaction between CD40 and gp39. Chi220 was used either alone or in combination with CTLA4-Ig in an immunosuppresive therapy study to check the survival of renal allografts in non human primate models (PMID: 12394833). Chi220 blocks binding of CD154, it also has partial agonist properties and weak stimulatory potential. It synergisticaly works with LEA29Y (belatacept) to prolong the islet allograft survival in a non-human primate Macaca fascicularis (PMID: 15611281). A study on protection of islets xenographs in diabetic macaques used a combination of Chi220 with IL-2 receptor specific antibody Basiliximab for immunosuppression with induction therapy. Chi220 is used as an sCD154 blocking agent and it was seen that it binds sCD154 in a dose dependant manner (PMID: 21920020). Two humanized versions of this antibody were generated F4 and L3.17, also showed similar properties. Humanized versions of this antibody are available on request.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: TNFSF5, CDw40, TRAP, p50, Bp50, CD40L receptor, Tumor necrosis factor ligand superfamily member 5, B-cell surface antigen CD40, T-cell antigen Gp39, TNF-related activation protein.

UniProt: P29965

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints