MSH6 antibody (AA 812-910) (Biotin)

Catalog No. ABIN704368

This Rabbit Polyclonal antibody specifically detects MSH6 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human and Rat.

Quick Overview for MSH6 antibody (AA 812-910) (Biotin) (ABIN704368)

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSH6 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MSH6 Antibody (Biotin)

Binding Specificity: AA 812-910

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MSH6

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Alternative Name: MSH6

Background:

Synonyms: GTBP, HSAP, p160, GTMBP, HNPCC5, DNA mismatch repair protein Msh6, hMSH6, G/T mismatch-binding protein, MutS-alpha 160 kDa subunit, MSH6

Background: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

Gene ID: 2956

UniProt: P52701

Pathways: DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response