Recombinant EpCAM antibody

Catalog No. ABIN7072687

Product Details anti-EpCAM Antibody

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This EpCAM antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Staining Methods (StM)

Purpose: Anti-EpCAM [KS1/4], Rabbit IgG, kappa

Specificity: This antibody binds to the EpCAM receptor which is expressed on surface of adenocarcinomas and almost all epithelial cell membranes except mesodermal and neural cell membranes.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with human adenocarcinoma of the lung cell line UCLA P3.

Clone: KS1-4

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been shown to be an effective agent for the invitro detection of disease and invivo diagnosis, prognosis and treatment of adenocarcinoma. Initial specificity of the antibody was determined by ELISA. Immunoperoxidase staining and immunofluorescence staining techniques were used to identify the reactivity of the antibody with various tissues (PMID: 6362852). The monoclonal antibody-vinca alkaloid conjugate, KS1/4-DAVLB (LY256787) were administered to nude mice bearing P3/UCLA human lung adenocarcinoma tumors. It was shown that KS1/4 monoclonal antibody targets DAVLB to the P3/UCLA human lung adenocarcinoma in vivo in the human xenograft model and that an increased therapeutic index may be achieved with LY256787 over conventional free drug therapy. (PMID: 3494841). KS1/4-DAVLB conjugates were also used for site directed therapy of epithelial malignancies (PMID: 2979063). Flow cytometric methods were used to evaluate of the cell surface binding properties of monoclonal antibody (KS1/4)-drμg/toxin conjugates (PMID: 3492560). Further characterization of the antibody via immunoperoxidase techniques, flow cytometric analyses and solid phase enzyme-linked immunoassays suggested that this antibody represents an epithelial malignancy marker (PMID: 3049306). Phase I clinical trials revealed that 10 out of eleven patients had human anti-mouse response (Elias et al., 1990). Humanized version of this antibody is available on request.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM (EPCAM Products)

Background: CD326, Ep-CAM, KS1/4 antigen, KSA, Epithelial cell adhesion molecule, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, 17-1A-antigen, EGP314, hEGP314, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1.

UniProt: P16422