Recombinant CD33 antibody

Catalog No. ABIN7072725

This anti-CD33 antibody is a Rabbit Chimeric antibody detecting CD33 in FACS, IF and RIA. Suitable for Human.

Quick Overview for Recombinant CD33 antibody (ABIN7072725)

Target: CD33

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD33 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Radioimmunoassay (RIA)

Clone: M195

Product Details anti-CD33 Antibody

Purpose: Anti-CD33 [M195], Rabbit IgG, kappa

Specificity: This antibody binds CD33, which is a 67 kD glycoprotein expressed on monocytes, hematopoetic progenitors, early myeloid progenitor cells and myeloid leukemias (acute and chronic) but not expressed on mature myeloid cells, normal stem cells, other hemtopoietic cells and non hematopoietic human tissues.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with live leukemic myeloblasts.

Isotype: IgG kappa

Application Details

Application Notes: This antibody is recommended for myeloid differentiation studies, diagnosis of acute non-lymphoid leukemia cells and therapeutic treatment of myelois leukemia. Specificity of this antibody to CD33 was determined using RIA and immunoabsorption. Immunoflurorescence assay showed that the antigen is not expressed on adult human tissues (PMID: 2716349). Flow cytometric analysis was used to confirm reactivity of M195 with fresh hematopoietic neoplasms in the blood or bone marrow. In combination with MY9, this antibody is useful in diagnosis of acute myelogenous leukemia(AML) (PMID: 2725060). Murine antibody M195 was used in the phase I trial of AML (PMID: 1999719). Humanized IgG1 and IgG3 versions of this antibody has higher apparent binding affinity for CD33 antigen than the chimeric and parent murine version (PMID: 1737932, 1458463). The increase in binding affinity resulted from eliminating an N-linked glycosylation site at residue 73 in the heavy chain variable region in the course of humanization (PMID: 8232322). Preliminary trials to treat AML with humanized antibody showed specific bone marrow targeting without an immunogenic response (PMID: 8124221).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD33

Target: CD33

Alternative Name: CD33

Background: Gp67, Siglec-3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3

UniProt: P20138