Recombinant LAG3 antibody

Catalog No. ABIN7072736

The Rat Monoclonal anti-LAG3 antibody has been validated for IP, FACS and BP. It is suitable to detect LAG3 in samples from Mouse.

Quick Overview for Recombinant LAG3 antibody (ABIN7072736)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LAG3 antibody is un-conjugated

Application: Immunoprecipitation (IP), Flow Cytometry (FACS), Blocking Peptide (BP)

Clone: C9B7W

Product Details anti-LAG3 Antibody

Purpose: Anti-LAG-3 [C9B7W], Rat IgG1, kappa

Specificity: This antibody is specific for an epitope in the D2 domain of murine LAG-3.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody has been used in flow cytometric analysis of splenocytes (Workman et al, 2005), T cell hybridomas expressing wild-type or chimeric CD4:murine LAG-3 (Workman et al, 2002) and CD4+ regulatory T cells (Chien et al, 2017). It has also been used in immunoprecipitation analysis of murine T hybridoma 3A9 cells transduced with murine LAG-3 (Li et al, 2004). This antibody blocks LAG-3 function in vitro, inhibiting antigen-induced IL-2 production in LAG-3-expressing T cell hybridomas, but does not block LAG-3:MHC class II interaction (Workman et al, 2002). Treatment with this antibody also blocks LAG-3 function in vivo, leading to accelerated homeostatic expansion of WT T cells (Workman et al, 2005). Administration of this LAG-3-blocking antibody, in combination with PD-L1 blockade, synergistically improves the survival of lymphodepleted myeloma-bearing mice (Jing et al, 2015).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: Lag3

Background: CD223, LAG3, Lymphocyte activation gene 3 protein

UniProt: Q61790

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints