CD74 antibody

Catalog No. ABIN7077436

The Mouse Monoclonal anti-CD74 antibody has been validated for FACS. It is suitable to detect CD74 in samples from Human.

Quick Overview for CD74 antibody (ABIN7077436)

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Grade: GMP Grade

Clone: PIN-1

Product Details anti-CD74 Antibody

Purpose: CD74 Unconjugated Antibody

Characteristics: PIN.1 recognizes a ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of the cytoplasmic tail of human CD74, a type II transmembrane glycoprotein. It is also known as invariant chain or MHC class II chaperone or MIF (macrophage migration inhibitory factor) receptor. It plays a critical role in the antigen processing leading to antigen presentation by MHC class II to CD4+ T cells. Antigen presenting cells such as B cells, monocytes/macrophages, dendritic cells, and Langerhans cells as well as activated T, carcinomas of lung, renal, gastric and thymic origins express CD74. The CD44 is an integral member of CD74 receptor complex and required for the CD74 signal transduction. CD74's cytoplasmic domain binds chromatin and regulates transcription and expression of genes involved in immune regulation, cell survival, and hematopoietic cancers. Published report claimed that the urease B subunit released by H. pylori binds to CD74 of gastric epithelial cells leading to induce NF-κB activation and IL-8 production.

Purification: Purified

Purity: >95 %

Immunogen: An EBV-transformed B cell line from a patient with MHC class II deficiency

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS pH 7.2, 0.1 % (w/v) BSA, 0.09 % (w/v) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74

Gene ID: 972

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints