UBR1 antibody (AA 801-900) (HRP)

Catalog No. ABIN704900

The Rabbit Polyclonal anti-UBR1 antibody has been validated for WB, ELISA, IHC (fro) and IHC (p). It is suitable to detect UBR1 in samples from Human and Mouse.

Quick Overview for UBR1 antibody (AA 801-900) (HRP) (ABIN704900)

Target: UBR1 (Ubiquitin Protein Ligase E3 Component N-Recognin 1 (UBR1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBR1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBR1 Antibody (HRP)

Binding Specificity: AA 801-900

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human eIF3A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UBR1

Target: UBR1 (Ubiquitin Protein Ligase E3 Component N-Recognin 1 (UBR1))

Alternative Name: e3A

Background:

Synonyms: EIF3, P167, p180, p185, TIF32, EIF3S10, eIF3-p170, eIF3-theta, Eukaryotic translation initiation factor 3 subunit A, eIF3a, Eukaryotic translation initiation factor 3 subunit 10, eIF-3-theta, eIF3 p167, eIF3 p180, eIF3 p185, KIAA0139

Background: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Gene ID: 8661

UniProt: Q14152