Amphiregulin antibody (AA 185-252)

Catalog No. ABIN704996

The Rabbit Polyclonal anti-Amphiregulin antibody has been validated for WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect Amphiregulin in samples from Human, Mouse, Rat and Guinea Pig. There are 3+ publications available.

Quick Overview for Amphiregulin antibody (AA 185-252) (ABIN704996)

Target: Amphiregulin (AREG)

Reactivity: Human, Mouse, Rat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Amphiregulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Amphiregulin Antibody

Binding Specificity: AA 185-252

Cross-Reactivity: Guinea Pig, Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Amphiregulin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Amphiregulin antibody (ABIN704996)

Jiang, Song, Li, Guo, Wu, Li, Guo, Shi, Bi, Jonas: "Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia." in: EBioMedicine, Vol. 17, pp. 134-144, (2017) (PubMed).

Keiler, Macejova, Dietz, Bolton, Pauli, Chen, van Breemen, Nikolic, Goerl, Muders, Zierau, Vollmer: "Evaluation of estrogenic potency of a standardized hops extract on mammary gland biology and on MNU-induced mammary tumor growth in rats." in: The Journal of steroid biochemistry and molecular biology, Vol. 174, pp. 234-241, (2017) (PubMed).

Liu, Fu, Song, Xue, Xia, Liu, Wang, Zhong, Li, Chen, Li, Cai, Li: "Mast Cells Participate in Corneal Development in Mice." in: Scientific reports, Vol. 5, pp. 17569, (2016) (PubMed).

Target Details for Amphiregulin

Target: Amphiregulin (AREG)

Alternative Name: Amphiregulin

Background:

Synonyms: AR, SDGF, AREGB, CRDGF, Amphiregulin, Colorectum cell-derived growth factor, AREG

Background: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Gene ID: 374

UniProt: P15514

Pathways: RTK Signaling, EGFR Signaling Pathway