ARHGEF12 antibody (AA 515-615)

Catalog No. ABIN705326

The Rabbit Polyclonal anti-ARHGEF12 antibody has been validated for ELISA, WB, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect ARHGEF12 in samples from Mouse.

Quick Overview for ARHGEF12 antibody (AA 515-615) (ABIN705326)

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ARHGEF12 Antibody

Binding Specificity: AA 515-615

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human LARG

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ARHGEF12

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Alternative Name: LARG

Background:

Synonyms: ARHGEF12, KIAA0382, leukemia-associated rho guanine nucleotide exchange factor, Leukemia-associated RhoGEF, PRO2792, Rho guanine exchange factor GEF 12, Rho guanine nucleotide exchange factor GEF 12, Rho guanine nucleotide exchange factor 12, Similar to Mouse Lsc oncogene, ARHGC_HUMAN.

Background: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein is observed to form myeloid/lymphoid fusion partner in acute myeloid leukemia (from EntrezGene). LARG has also been implicated in linking heterotrimeric G proteins to microtubule organisation in regulation of cell polarity.

Gene ID: 23365

Pathways: Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling