Sphingomyelin Synthase 1 antibody (AA 331-413) (Cy5)

Catalog No. ABIN705375

This anti-Sphingomyelin Synthase 1 antibody is a Rabbit Polyclonal antibody detecting Sphingomyelin Synthase 1 in WB, IF (cc) and IF (p). Suitable for Mouse.

Quick Overview for Sphingomyelin Synthase 1 antibody (AA 331-413) (Cy5) (ABIN705375)

Target: Sphingomyelin Synthase 1 (SGMS1)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sphingomyelin Synthase 1 antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Sphingomyelin Synthase 1 Antibody (Cy5)

Binding Specificity: AA 331-413

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Sphingomyelin Synthase 1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Sphingomyelin Synthase 1

Target: Sphingomyelin Synthase 1 (SGMS1)

Alternative Name: Sphingomyelin Synthase 1

Background:

Synonyms: MOB, MOB1, SMS1, TMEM23, hmob33, Phosphatidylcholine:ceramide cholinephosphotransferase 1, Medulla oblongata-derived protein, Protein Mob, Sphingomyelin synthase 1, Transmembrane protein 23, SGMS1

Background: Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types such as HeLa cells. Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide.

Gene ID: 259230

UniProt: Q86VZ5

Pathways: Cellular Response to Molecule of Bacterial Origin