MED1 antibody (AA 1321-1420)

Catalog No. ABIN705476

Product Details anti-MED1 Antibody

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Binding Specificity: AA 1321-1420

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TRAP220/MED1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MED1

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Alternative Name: TRAP220 (MED1 Products)

Background:

Synonyms: CRSP1, CRSP200, DRIP205, DRIP230, MED1, MED1_HUMAN, Mediator complex subunit 1, Mediator of RNA polymerase II transcription subunit 1, p53 regulatory protein RB18A, PBP, Peroxisome prolerator-activated receptor-binding protein, PPAR binding protein, PPAR-binding protein, PPARBP, PPARGBP, RB18A, Thyroid hormone receptor-associated protein complex 220 kDa component, Thyroid receptor-interacting protein 2, TR-interacting protein 2, TRAP220, TRIP-2, TRIP2, Vitamin D receptor-interacting protein complex component DRIP205, Activator-recruited cofactor 205 kDa component, ARC205.

Background: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene ID: 5469

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha