PHD1 antibody (AA 321-407) (Cy5.5)

Catalog No. ABIN705901

This Rabbit Polyclonal antibody specifically detects PHD1 in WB, IF (cc) and IF (p). It exhibits reactivity toward Mouse.

Quick Overview for PHD1 antibody (AA 321-407) (Cy5.5) (ABIN705901)

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHD1 antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-PHD1 Antibody (Cy5.5)

Binding Specificity: AA 321-407

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PHD1/prolyl hydroxylase

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PHD1

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Alternative Name: PHD1

Background:

Synonyms: Egl nine homolog 2, prolyl hydroxylase,EGLN 2, EGLN2, EIT 6, EIT6, H P4H 1, H PH1, HPH1, HPH 3, HPH3, P4H1, PHD 1, PHD1, EGL nine C.elegans homolog 2, EGLN2_HUMAN.

Background: PHD1 catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins and hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation.

Gene ID: 112398

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis