EPAS1 antibody (AA 471-570)

Catalog No. ABIN7091058

This Rabbit Polyclonal antibody specifically detects EPAS1 in ELISA and IHC (p). It exhibits reactivity toward Rat.

Quick Overview for EPAS1 antibody (AA 471-570) (ABIN7091058)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EPAS1 Antibody

Binding Specificity: AA 471-570

Cross-Reactivity: Rat

Predicted Reactivity: Human,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HIF 2 alpha

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: HIF 2 alpha

Background:

Synonyms: Basic helix loop helix PAS protein MOP2, Basic-helix-loop-helix-PAS protein MOP2, bHLHe73, Class E basic helix-loop-helix protein 73, ECYT4, Endothelial PAS domain containing protein 1, Endothelial pas domain protein 1, Endothelial PAS domain-containing protein 1, EPAS 1, EPAS-1, EPAS1, EPAS1_HUMAN, HIF 1 alpha like factor, HIF 2 alpha, HIF-1-alpha-like factor, HIF-2-alpha, HIF2-alpha, HIF2A, HLF, Hypoxia inducible factor 2 alpha, Hypoxia inducible factor 2 alpha subunit, Hypoxia-inducible factor 2-alpha, Member of PAS protein 2, Member of pas superfamily 2, MOP 2, MOP2, PAS domain-containing protein 2, PASD2.

Background: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

Gene ID: 2034

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect