EIF2S1 antibody

Catalog No. ABIN7091086

The Rabbit Monoclonal anti-EIF2S1 antibody is suitable to detect EIF2S1 in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for EIF2S1 antibody (ABIN7091086)

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EIF2S1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: F7A11

Product Details anti-EIF2S1 Antibody

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EIF2S1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for EIF2S1

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Alternative Name: EIF2S1

Background:

Synonyms: EIF2A, CDA 02, CDA02, eIF 2 alpha, EIF 2, EIF 2A, EIF-2alpha, EIF2, EIF2alpha, eIF2S1, Eukaryotic Translation Initiation Factor 2 alpha, Eukaryotic translation initiation factor 2 subunit 1 alpha 35 kDa, Eukaryotic translation initiation factor 2 subunit alpha, IF2A_HUMAN.

Background: eIF2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF2 alpha is phosphorylated at serine 51 (human EIF2 alpha, the equivalent residue in mouse is serine 52) by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important regulatory process in protein synthesis.

Gene ID: 1965

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C