LIN28B antibody

Catalog No. ABIN7091335

The Mouse Monoclonal anti-LIN28B antibody is suitable to detect LIN28B in samples from Human. It has been validated for WB.

Quick Overview for LIN28B antibody (ABIN7091335)

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIN28B antibody is un-conjugated

Application: Western Blotting (WB)

Clone: K7L3

Product Details anti-LIN28B Antibody

Predicted Reactivity: Human

Immunogen: Recombinant human LIN28B.

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for LIN28B

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Alternative Name: LIN28B

Background:

Synonyms: Lin-28B, LN28B_HUMAN, CSDD 2, CSDD2, FLJ16517, Lin 28 homolog B (C. elegans), Lin 28.2, Lin-28B, Protein lin-28 homolog B.

Background: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Gene ID: 389421

UniProt: Q6ZN17