Pol II antibody

Catalog No. ABIN7091532

This Mouse Monoclonal antibody specifically detects in IF and WB. It exhibits reactivity toward Human.

Quick Overview for Pol II antibody (ABIN7091532)

Target: Pol II

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), Western Blotting (WB)

Product Details

Cross-Reactivity: Human

Purification: Purified by Protein G.

Immunogen: Monoclonal antibody raised in mouse against the YSPTSPS repeat in the B1 subunit of RNA polymerase II

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IF()

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: PBS with 0.05 % sodium azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details

Target: Pol II

Background:

Synonyms: POLR2A, RPB1, POLR2, RPOL2

Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression

Gene ID: 5430

UniProt: P24928