gamma Tubulin antibody (AbBy Fluor® 750)

Catalog No. ABIN7091786

The AbBy Fluor® 750-conjugated Mouse Monoclonal anti-gamma Tubulin antibody (Clone 8D11) (ABIN7091786) specifically detects gamma Tubulin in WB and IF (p). The antibody is reactive with Mouse, Human and Rat samples.

Quick Overview for gamma Tubulin antibody (AbBy Fluor® 750) (ABIN7091786)

Target: gamma Tubulin (TUBG) (Tubulin, gamma (TUBG))

Reactivity: Mouse, Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This gamma Tubulin antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: 8D11

Product Details anti-gamma Tubulin Antibody (AbBy Fluor® 750)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein G.

Immunogen: KLH conjugated synthetic peptide derived from human Gamma Tubulin

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Target Details for gamma Tubulin

Target: gamma Tubulin (TUBG) (Tubulin, gamma (TUBG))

Alternative Name: Gamma Tubulin

Background:

Synonyms: Centrosome Marker, Gamma 1 tubulin, gamma tubulin, gamma-tubulin, Gamma 2 tubulin, Gamma Tubulin 1, Gamma Tubulin 2, Gamma tubulin complex component 1, GCP 1, GCP-1, GCP1, MGC131994, TUBG, TUBG1, TUBG2, Tubulin gamma 1 chain, Tubulin gamma 2 chain, Xgam, TBG1_HUMAN, TBG2_HUMAN, Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1.

Background: Centrosome Marker
Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end.
The abundance of Gamma tubulin is less than 1 % of the level of either alpha or beta tubulin. It shares approximately 28-32 % identity with alpha tubulin from various organisms and 32-36 % identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.

Gene ID: 7283

UniProt: P23258