ALKBH5 antibody

Catalog No. ABIN7111483

This Rabbit Polyclonal antibody specifically detects ALKBH5 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ALKBH5 antibody (ABIN7111483)

Target: ALKBH5 (AlkB, Alkylation Repair Homolog 5 (ALKBH5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-ALKBH5 Antibody

Purpose: ALKBH5 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: alkB, alkylation repair homolog 5(E. coli)

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ALKBH5

Target: ALKBH5 (AlkB, Alkylation Repair Homolog 5 (ALKBH5))

Alternative Name: ALKBH5

Background:

Synonyms: RNA demethylase ALKBH5|Alkylated DNA repair protein alkB homolog 5|Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5|ALKBH5|ABH5|OFOXD1

Background: Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine(m6A) RNA, the most prevalent internal modification of messenger RNA(mRNA) in higher eukaryotes(PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Can also demethylate N(6)-methyladenosine in single-stranded DNA(in vitro)(PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron(PubMed:21264265, PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export(PubMed:23177736). Required for spermatogenesis(By similarity).

Molecular Weight: 51 kDa

Gene ID: 54890

UniProt: Q6P6C2