ACTN1 antibody (AA 272-490)

Catalog No. ABIN7111505

The Rabbit Polyclonal anti-ACTN1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect ACTN1 in samples from Human, Mouse, Rat and Monkey.

Quick Overview for ACTN1 antibody (AA 272-490) (ABIN7111505)

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-ACTN1 Antibody

Binding Specificity: AA 272-490

Purpose: ACTN1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 272-490aa

Immunogen: actinin, alpha 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ACTN1

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Alternative Name: ACTN1

Background:

Synonyms: Alpha-actinin-1|Alpha-actinin cytoskeletal isoform|F-actin cross-linking protein|Non-muscle alpha-actinin-1|ACTN1

Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 110 kDa

Gene ID: 87

UniProt: P12814

Pathways: Cell-Cell Junction Organization