APP antibody

Catalog No. ABIN7111534

This Mouse Monoclonal antibody specifically detects APP in WB, ELISA and IHC. It exhibits reactivity toward Human and Pig.

Quick Overview for APP antibody (ABIN7111534)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 8B9

Product Details anti-APP Antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: amyloid beta A4 precursor protein

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: APP

Background: Synonyms:A4, ABPP,Alzheimer disease amyloid protein,Cerebral vascular amyloid peptide,Beta-amyloid precursor protein, CVAP,PreA4 Background:Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Beta-amyloid 42 is a more effective reductant than beta-amyloid 40. Beta-amyloid peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts. The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.

Molecular Weight: 100 kDa

Gene ID: 351

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour