ATP5F1A (AA 200-553) antibody

Catalog No. ABIN7111797

The Mouse Monoclonal anti-ATP5F1A antibody (Clone 9G0) (ABIN7111797) specifically detects ATP5F1A in ELISA, WB, IHC, IF and FACS. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for ATP5F1A (AA 200-553) antibody (ABIN7111797)

Target: ATP5F1A

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 9G0

Product Details

Binding Specificity: AA 200-553

Purpose: ATP5F1A antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 200-553aa

Immunogen: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:500-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details

Target: ATP5F1A

Background:

Synonyms: ATP synthase subunit alpha, mitochondrial|ATP synthase F1 subunit alpha|ATP5F1A|ATP5A|ATP5A1|ATP5AL2|ATPM

Background: Mitochondrial membrane ATP synthase(F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1)-containing the extramembraneous catalytic core, and F(0)-containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites(By similarity).

Molecular Weight: 50-55 kDa

Gene ID: 498

UniProt: P25705