ATP5F1C (AA 1-298) antibody

Catalog No. ABIN7111799

The Mouse Monoclonal anti- antibody has been validated for ELISA, WB and IHC. It is suitable to detect in samples from Human.

Quick Overview for ATP5F1C (AA 1-298) antibody (ABIN7111799)

Target: ATP5F1C

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 8H11

Product Details

Binding Specificity: AA 1-298

Purpose: ATP5F1C antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-298aa

Immunogen: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details

Target: ATP5F1C

Background:

Synonyms: ATP synthase subunit gamma, mitochondrial|ATP synthase F1 subunit gamma|F-ATPase gamma subunit|ATP5F1C|ATP5C|ATP5C1|ATP5CL1

Background: Mitochondrial membrane ATP synthase(F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1)-containing the extramembraneous catalytic core, and F(0)-containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Molecular Weight: 33 kDa

Gene ID: 509

UniProt: P36542