CDK5RAP3 antibody

Catalog No. ABIN7112544

This anti-CDK5RAP3 antibody is a Rabbit Polyclonal antibody detecting CDK5RAP3 in WB, ELISA, IHC, IF and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for CDK5RAP3 antibody (ABIN7112544)

Target: CDK5RAP3 (CDK5 Regulatory Subunit Associated Protein 3 (CDK5RAP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK5RAP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-CDK5RAP3 Antibody

Purpose: CDK5RAP3 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: CDK5 regulatory subunit associated protein 3

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for CDK5RAP3

Target: CDK5RAP3 (CDK5 Regulatory Subunit Associated Protein 3 (CDK5RAP3))

Alternative Name: CDK5RAP3

Background:

Synonyms: CDK5 regulatory subunit-associated protein 3|CDK5 activator-binding protein C53|LXXLL/leucine-zipper-containing ARF-binding protein|Protein HSF-27|CDK5RAP3|IC53|LZAP

Background: Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation(PubMed:12054757, PubMed:12737517). Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation(PubMed:17785205, PubMed:20228063). Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint(PubMed:15790566, PubMed:19223857). Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation(PubMed:16173922). May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress(PubMed:23152784). May also play a role in the rupture of the nuclear envelope during apoptosis(PubMed:23478299). May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1(PubMed:21283629). (Microbial infection) May be negatively regulated by hepatitis B virus large envelope protein mutant pre-s2 to promote mitotic entry.

Molecular Weight: 65 kDa

Gene ID: 80279

UniProt: Q96JB5