CPSF3 antibody

Catalog No. ABIN7112880

This anti-CPSF3 antibody is a Rabbit Polyclonal antibody detecting CPSF3 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for CPSF3 antibody (ABIN7112880)

Target: CPSF3 (Cleavage and Polyadenylation Specific Factor 3, 73kDa (CPSF3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPSF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CPSF3 Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: cleavage and polyadenylation specific factor 3, 73kDa

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for CPSF3

Target: CPSF3 (Cleavage and Polyadenylation Specific Factor 3, 73kDa (CPSF3))

Alternative Name: CPSF3

Background: Synonyms:CPSF73 Background:Component of the cleavage and polyadenylation specificity factor(CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product(DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop(SL) and 5' phosphate on the downstream cleavage product(DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product(DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.

Molecular Weight: 77kd

Gene ID: 51692

UniProt: Q9UKF6