DBT antibody

Catalog No. ABIN7113483

This anti-DBT antibody is a Rabbit Polyclonal antibody detecting DBT in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for DBT antibody (ABIN7113483)

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DBT Antibody

Purpose: DBT antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: dihydrolipoamide branched chain transacylase E2

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for DBT

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Alternative Name: DBT

Background:

Synonyms: Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial|52 kDa mitochondrial autoantigen of primary biliary cirrhosis|Branched chain 2-oxo-acid dehydrogenase complex component E2 (BCOADC-E2)|Branched-chain alpha-keto acid dehydrogenase complex component E2 (BCKAD-E2, BCKADE2, BCKDH-E2)|Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex|Dihydrolipoamide branched chain transacylase|Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase|DBT|BCATE2|BCKDHE2

Background: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase(E1), lipoamide acyltransferase(E2) and lipoamide dehydrogenase(E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.

Molecular Weight: 53 kDa

Gene ID: 1629

UniProt: P11182