DDIT3 antibody (AA 1-169)

Catalog No. ABIN7113561

The Mouse Monoclonal anti-DDIT3 antibody (Clone 5H7) (ABIN7113561) specifically detects DDIT3 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for DDIT3 antibody (AA 1-169) (ABIN7113561)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This DDIT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 5H7

Product Details anti-DDIT3 Antibody

Binding Specificity: AA 1-169

Purpose: DDIT3 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-169aa

Immunogen: DNA-damage-inducible transcript 3

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Alternative Name: DDIT3

Background:

Synonyms: DNA damage-inducible transcript 3 protein (DDIT-3)|C/EBP zeta|C/EBP-homologous protein (CHOP)|C/EBP-homologous protein 10 (CHOP-10)|CCAAT/enhancer-binding protein homologous protein|Growth arrest and DNA damage-inducible protein GADD153|DDIT3|CHOP|CHOP10|GADD153

Background: CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions to hamster gene.Imposed by unfolded and malfolded proteins, CHOP is significantly induced by ER stress, deficiency of CHOP prevents cell from ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP by forming a heterodimer. It may play an important role in the malignant transformation of nevus to melanoma.

Molecular Weight: 30 kDa

Gene ID: 1649

UniProt: P35638

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis