EGLN3 antibody

Catalog No. ABIN7113751

This Rabbit Polyclonal antibody specifically detects EGLN3 in WB, ELISA, IHC and IP. It exhibits reactivity toward Human.

Quick Overview for EGLN3 antibody (ABIN7113751)

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGLN3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-EGLN3 Antibody

Purpose: EGLN3 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: egl nine homolog 3

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:500-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for EGLN3

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Alternative Name: EGLN3

Background:

Synonyms: Prolyl hydroxylase EGLN3|Egl nine homolog 3|HPH-1|Hypoxia-inducible factor prolyl hydroxylase 3 (HIF-PH3, HIF-prolyl hydroxylase 3, HPH-3)|Prolyl hydroxylase domain-containing protein 3 (PHD3)|EGLN3

Background: EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor(HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation(ODD) domains(N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

Molecular Weight: 28 kDa

UniProt: Q9H6Z9

Pathways: Positive Regulation of Endopeptidase Activity