EIF3D antibody

Catalog No. ABIN7113953

The Rabbit Polyclonal anti-EIF3D antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect EIF3D in samples from Human, Mouse and Saccharomyces cerevisiae.

Quick Overview for EIF3D antibody (ABIN7113953)

Target: EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))

Reactivity: Human, Mouse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-EIF3D Antibody

Purpose: EIF3D antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: eukaryotic translation initiation factor 3, subunit D

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for EIF3D

Target: EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))

Alternative Name: EIF3D

Background:

Synonyms: Eukaryotic translation initiation factor 3 subunit D (eIF3d)|Eukaryotic translation initiation factor 3 subunit 7|eIF-3-zeta|eIF3 p66|EIF3D|EIF3S7

Background: Component of the eukaryotic translation initiation factor 3(eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex(43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2(PubMed:18056426).

Molecular Weight: 66 kDa

Gene ID: 8664

UniProt: O15371

Pathways: Ribonucleoprotein Complex Subunit Organization