EIF3G antibody

Catalog No. ABIN7113955

This Rabbit Polyclonal antibody specifically detects EIF3G in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EIF3G antibody (ABIN7113955)

Target: EIF3G (Eukaryotic Translation Initiation Factor 3, Subunit G (EIF3G))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3G antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EIF3G Antibody

Purpose: EIF3G antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: eukaryotic translation initiation factor 3, subunit G

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for EIF3G

Target: EIF3G (Eukaryotic Translation Initiation Factor 3, Subunit G (EIF3G))

Alternative Name: EIF3G

Background:

Synonyms: Eukaryotic translation initiation factor 3 subunit G (eIF3g)|Eukaryotic translation initiation factor 3 RNA-binding subunit (eIF-3 RNA-binding subunit)|Eukaryotic translation initiation factor 3 subunit 4|eIF-3-delta|eIF3 p42|eIF3 p44|EIF3G|EIF3S4

Background: Component of the eukaryotic translation initiation factor 3(eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex(43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA. In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2(PubMed:18056426).

Molecular Weight: 44 kDa

Gene ID: 8666

UniProt: O75821

Pathways: Ribonucleoprotein Complex Subunit Organization