EIF4A3 antibody

Catalog No. ABIN7113966

This anti-EIF4A3 antibody is a Rabbit Polyclonal antibody detecting EIF4A3 in WB, ELISA, IHC, IP and IF. Suitable for Human, Mouse and Rat.

Quick Overview for EIF4A3 antibody (ABIN7113966)

Target: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-EIF4A3 Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: eukaryotic translation initiation factor 4A, isoform 3

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for EIF4A3

Target: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Alternative Name: EIF4A3

Background: Synonyms:DDX48, KIAA0111 Background:ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex(EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay(NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X(and probably other apoptotic genes), specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S), the function is different from the established EJC assembly. Involved in craniofacial development.

Molecular Weight: 47 kDa

Gene ID: 9775

UniProt: P38919