ERI1 antibody

Catalog No. ABIN7114005

This anti-ERI1 antibody is a Rabbit Polyclonal antibody detecting ERI1 in WB, ELISA, IHC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for ERI1 antibody (ABIN7114005)

Target: ERI1 (Exoribonuclease 1 (ERI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ERI1 Antibody

Purpose: ERI1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: exoribonuclease 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ERI1

Target: ERI1 (Exoribonuclease 1 (ERI1))

Alternative Name: ERI1

Background:

Synonyms: 3'-5' exoribonuclease 1|3'-5' exonuclease ERI1|Eri-1 homolog|Histone mRNA 3'-end-specific exoribonuclease|Histone mRNA 3'-exonuclease 1|Protein 3'hExo (HEXO)|ERI1|3'EXO|THEX1

Background: RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs(siRNAs) in vitro, suggesting a possible role as regulator of RNA interference(RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

Molecular Weight: 46 kDa

Gene ID: 90459

UniProt: Q8IV48