ERI1 antibody (N-Term)

Catalog No. ABIN2776656

Cited in 1 publication. The Rabbit Polyclonal anti-ERI1 antibody (ABIN2776656) specifically detects ERI1 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse and Pig samples.

Quick Overview for ERI1 antibody (N-Term) (ABIN2776656)

Target: ERI1 (Exoribonuclease 1 (ERI1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ERI1 Antibody

Binding Specificity: N-Term

Purpose: ERI1 Antibody - N-terminal region

Sequence: SKFITSSASD FSDPVYKEIA ITNGCINRMS KEELRAKLSE FKLETRGVKD

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 85%, Pig: 93%, Rabbit: 100%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against THEX1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ERI1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8100070

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ERI1 antibody (ABIN2776656)

Kupsco, Wu, Marzluff, Thapar, Duronio: "Genetic and biochemical characterization of Drosophila Snipper: A promiscuous member of the metazoan 3'hExo/ERI-1 family of 3' to 5' exonucleases." in: RNA (New York, N.Y.), Vol. 12, Issue 12, pp. 2103-17, (2006) (PubMed).

Target Details for ERI1

Target: ERI1 (Exoribonuclease 1 (ERI1))

Alternative Name: ERI1

Background:

Background Information: RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

Gene Name: exoribonuclease 1

Alternative Symbols: HEXO, THEX1, 3'HEXO

Protein Name: 3'-5' exoribonuclease 1

Molecular Weight: 349

Gene ID: 90459

NCBI Accession: NP_699163

UniProt: Q8IV48