F-Box DNA Helicase 1 (FBXO18) antibody

Catalog No. ABIN7114057

This Rabbit Polyclonal antibody specifically detects in ELISA and WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for F-Box DNA Helicase 1 (FBXO18) antibody (ABIN7114057)

Target: F-Box DNA Helicase 1 (FBXO18)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Product Details

Purpose: FBH1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: F-box protein, helicase, 18

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details

Target: F-Box DNA Helicase 1 (FBXO18)

Alternative Name: FBH1

Background:

Synonyms: F-box DNA helicase 1 (hFBH1)|DNA 3'-5' helicase 1|F-box only protein 18|FBH1|FBX18|FBXO18

Background: 3'-5' DNA helicase and substrate-recognition component of the SCF(FBXO18) E3 ubiquitin ligase complex that plays a key role in response to stalled/damaged replication forks(PubMed:11956208, PubMed:23393192). Involved in genome maintenance by acting as an anti-recombinogenic helicase and preventing extensive strand exchange during homologous recombination: promotes RAD51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination(PubMed:17724085, PubMed:19736316). Also promotes cell death and DNA double-strand breakage in response to replication stress: together with MUS81, promotes the endonucleolytic DNA cleavage following prolonged replication stress via its helicase activity, possibly to eliminate cells with excessive replication stress(PubMed:23319600, PubMed:23361013). Plays a major role in remodeling of stalled DNA forks by catalyzing fork regression, in which the fork reverses and the two nascent DNA strands anneal(PubMed:25772361). In addition to the helicase activity, also acts as the substrate-recognition component of the SCF(FBXO18) E3 ubiquitin ligase complex, a complex that mediates ubiquitination of RAD51, leading to regulate RAD51 subcellular location(PubMed:25585578).

Molecular Weight: 118-123 kDa

Gene ID: 84893

UniProt: Q8NFZ0