FOXA2 antibody

Catalog No. ABIN7114288

This Rabbit Polyclonal antibody specifically detects FOXA2 in WB, ELISA, IHC and IP. It exhibits reactivity toward Human.

Quick Overview for FOXA2 antibody (ABIN7114288)

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-FOXA2 Antibody

Purpose: FOXA2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: forkhead box A2

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for FOXA2

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Alternative Name: FOXA2

Background:

Synonyms: Hepatocyte nuclear factor 3-beta (HNF-3-beta, HNF-3B)|Forkhead box protein A2|Transcription factor 3B (TCF-3B)|FOXA2|HNF3B|TCF3B

Background: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3'(By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs, FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis, regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.

Molecular Weight: 60 kDa

Gene ID: 3170

UniProt: Q9Y261

Pathways: Dopaminergic Neurogenesis, Regulation of Carbohydrate Metabolic Process