HPSE antibody

Catalog No. ABIN7114948

This Rabbit Polyclonal antibody specifically detects HPSE in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HPSE antibody (ABIN7114948)

Target: HPSE (Heparanase (HPSE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPSE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-HPSE Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: heparanase

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for HPSE

Target: HPSE (Heparanase (HPSE))

Alternative Name: HPSE

Background: Synonyms:HEP, HPA, HPA1, HPR1, HPSE1, HSE1 Background:Endoglycosidase that cleaves heparan sulfate proteoglycans(HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix(ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix(ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.

Molecular Weight: 60 kDa

Gene ID: 10855

UniProt: Q9Y251

Pathways: Glycosaminoglycan Metabolic Process