HINT1 antibody

Catalog No. ABIN7115028

The Rabbit Polyclonal anti-HINT1 antibody (ABIN7115028) specifically detects HINT1 in WB, ELISA, IHC and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for HINT1 antibody (ABIN7115028)

Target: HINT1 (Histidine Triad Nucleotide Binding Protein 1 (HINT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HINT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-HINT1 Antibody

Purpose: HINT1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: histidine triad nucleotide binding protein 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for HINT1

Target: HINT1 (Histidine Triad Nucleotide Binding Protein 1 (HINT1))

Alternative Name: HINT1

Background:

Synonyms: Adenosine 5'-monophosphoramidase HINT1|Desumoylating isopeptidase HINT1|Histidine triad nucleotide-binding protein 1|Protein kinase C inhibitor 1|Protein kinase C-interacting protein 1 (PKCI-1)|HINT1|HINT|PKCI1|PRKCNH1

Background: Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate(AMP-NH2), adenosine 5'monophosphomorpholidate(AMP-morpholidate) and guanosine 5'monophosphomorpholidate(GMP-morpholidate). Hydrolyzes lysyl-AMP(AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP(GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF(SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex.

Molecular Weight: 14 kDa

Gene ID: 3094

UniProt: P49773