ITK antibody

Catalog No. ABIN7115182

This Rabbit Polyclonal antibody specifically detects ITK in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ITK antibody (ABIN7115182)

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ITK Antibody

Purpose: ITK antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: IL2-inducible T-cell kinase

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: ITK

Background:

Synonyms: Tyrosine-protein kinase ITK/TSK|Interleukin-2-inducible T-cell kinase (IL-2-inducible T-cell kinase)|Kinase EMT|T-cell-specific kinase|Tyrosine-protein kinase Lyk|ITK|EMT|LYK

Background: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells(APC) activate T-cell receptor(TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells(NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation.

Molecular Weight: 68-72 kDa

Gene ID: 3702

UniProt: Q08881

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway