Lamin B1 antibody

Catalog No. ABIN7115761

This Mouse Monoclonal antibody specifically detects Lamin B1 in WB, ELISA, IP, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Lamin B1 antibody (ABIN7115761)

Target: Lamin B1 (LMNB1)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 4A4

Product Details anti-Lamin B1 Antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: lamin B1

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:5000-1:50000 , IHC: 1:500-1:2000, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for Lamin B1

Target: Lamin B1 (LMNB1)

Alternative Name: Lamin B1

Background: Synonyms:ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1 Background:Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.

Molecular Weight: 66kd

UniProt: P20700

Pathways: Apoptosis, Caspase Cascade in Apoptosis