MED30 antibody

Catalog No. ABIN7116129

The Rabbit Polyclonal anti-MED30 antibody (ABIN7116129) specifically detects MED30 in WB, ELISA and IP. The antibody is reactive with Human and Mouse samples.

Quick Overview for MED30 antibody (ABIN7116129)

Target: MED30 (Mediator Complex Subunit 30 (MED30))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED30 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-MED30 Antibody

Purpose: MED30 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: mediator complex subunit 30

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for MED30

Target: MED30 (Mediator Complex Subunit 30 (MED30))

Alternative Name: MED30

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 30|Mediator complex subunit 30|TRAP/Mediator complex component TRAP25|Thyroid hormone receptor-associated protein 6|Thyroid hormone receptor-associated protein complex 25 kDa component (Trap25)|MED30|THRAP6|TRAP25

Background: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Molecular Weight: 20-25 kDa

Gene ID: 90390

UniProt: Q96HR3

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha