MTF2 antibody

Catalog No. ABIN7116220

This Rabbit Polyclonal antibody specifically detects MTF2 in WB, ELISA, IF, IHC and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MTF2 antibody (ABIN7116220)

Target: MTF2 (Metal Response Element Binding Transcription Factor 2 (MTF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-MTF2 Antibody

Purpose: MTF2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: metal response element binding transcription factor 2

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for MTF2

Target: MTF2 (Metal Response Element Binding Transcription Factor 2 (MTF2))

Alternative Name: MTF2

Background:

Synonyms: Metal-response element-binding transcription factor 2|Metal regulatory transcription factor 2|Metal-response element DNA-binding protein M96|Polycomb-like protein 2 (hPCl2)|MTF2|PCL2

Background: Polycomb group(PcG) that specifically binds histone H3 trimethylated at 'Lys-36'(H3K36me3) and recruits the PRC2 complex. Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element(MRE) of MT1A gene promoter(By similarity).

Molecular Weight: 60-61 kDa

Gene ID: 22823

UniProt: Q9Y483

Pathways: Stem Cell Maintenance