METAP2 antibody

Catalog No. ABIN7116239

The Rabbit Polyclonal anti-METAP2 antibody is suitable to detect METAP2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for METAP2 antibody (ABIN7116239)

Target: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This METAP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-METAP2 Antibody

Purpose: METAP2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: methionyl aminopeptidase 2

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for METAP2

Target: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Alternative Name: METAP2

Background:

Synonyms: Methionine aminopeptidase 2 (MAP 2, MetAP 2)|Initiation factor 2-associated 67 kDa glycoprotein (p67, p67eIF2)|Peptidase M|METAP2|MNPEP|P67EIF2

Background: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged(Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.

Molecular Weight: 67 kDa

Gene ID: 10988

UniProt: P50579

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling